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plasmid pet26b  (Addgene inc)


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    Addgene inc plasmid pet26b
    Plasmid Pet26b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet26b+plasmid/pET26b-SET+(Plasmid+%238822)/pm41599394-103-6-8
    Average 92 stars, based on 4 article reviews
    plasmid pet26b - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A HER2 bispecific antibody can be efficiently expressed in Escherichia coli with potent cytotoxicity
    Article Snippet: Plasmids To make the CD3-S-Fab bispecific antibody, the VH-CH1 and VL-CL of anti-CD3 UCHT1 clone ( 31 ) were synthesized (Genscript Biotech., Nanjing, China). .. The VH-CH1 was cloned into the pET26b plasmid (Addgene, Inc., Cambridge, MA, USA) through restriction enzyme cutting site Nco I and BamH I ( ). .. The VL-CL of anti-CD3 UCHT1 was linked with the single domain anti-HER2 VHH ( 8 ), and then cloned into the pET21a (Addgene, Cambridge, MA, USA) through restriction enzyme cutting site Nco I and Xho I ( ).

    Plasmid Preparation:

    Article Title: A HER2 bispecific antibody can be efficiently expressed in Escherichia coli with potent cytotoxicity
    Article Snippet: Plasmids To make the CD3-S-Fab bispecific antibody, the VH-CH1 and VL-CL of anti-CD3 UCHT1 clone ( 31 ) were synthesized (Genscript Biotech., Nanjing, China). .. The VH-CH1 was cloned into the pET26b plasmid (Addgene, Inc., Cambridge, MA, USA) through restriction enzyme cutting site Nco I and BamH I ( ). .. The VL-CL of anti-CD3 UCHT1 was linked with the single domain anti-HER2 VHH ( 8 ), and then cloned into the pET21a (Addgene, Cambridge, MA, USA) through restriction enzyme cutting site Nco I and Xho I ( ).

    Article Title: Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI
    Article Snippet: .. 85732, 85736 and 85737, respectively) SpyCatcher-mNeonGreen in pET26b plasmid (https://www.addgene. org/vector-database/9135) pST39 plasmid containing pNL29 Mega GV gene cluster (Addgene, ID no. 91696) SOC outgrowth medium (NEB, cat. no. B9020S) Luria Broth (LB) base (Miller’s LB Broth Base), powder (Invitrogen; Life Technologies, cat. no.12795-084) Ampicillin sodium salt (Sigma-Aldrich, cat. no. A0166-25G) Chloramphenicol (Sigma-Aldrich, cat. no. C0378) D-(+)-Glucose (Sigma-Aldrich, cat. no.G8270) Glycerol (Sigma-Aldrich, cat. no.G5516) PBS, 20× solution (Teknova, cat. no. P1225) SCID Hairless Outbred (SHO) or SCID Hairless Congenic (SHC) mice, female, 4–6 weeks of age (Charles River, cat. no. 474 or 488) ! cautIon All procedures involving animal use must be performed in accordance with institutional guidelines and regulations and approved by the relevant animal care and use committees. ..

    Article Title: Investigating the Importance of the Beta-Octyl Glucoside Binding Site for the Toxicity of C. Perfringens Epsilon Toxin
    Article Snippet: .. The pET26b(+) plasmid was the only vector used for the e—toxin mutagenesis study (Addgene plasmid # 69862-3). .. 8.2.2 Oligonucleotide Primer Design Mutagenic primers for e—toxin were designed using the QuikChange Primer Design Program.

    Mutagenesis:

    Article Title: Investigating the Importance of the Beta-Octyl Glucoside Binding Site for the Toxicity of C. Perfringens Epsilon Toxin
    Article Snippet: .. The pET26b(+) plasmid was the only vector used for the e—toxin mutagenesis study (Addgene plasmid # 69862-3). .. 8.2.2 Oligonucleotide Primer Design Mutagenic primers for e—toxin were designed using the QuikChange Primer Design Program.



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    Endotoxin levels and yields of variable domain of the heavy chain antibodies produced by the endotoxin-free and Escherichia coli systems.

    Journal: PNAS Nexus

    Article Title: Development of a novel bacterial production system for recombinant bioactive proteins completely free from endotoxin contamination

    doi: 10.1093/pnasnexus/pgae328

    Figure Lengend Snippet: Endotoxin levels and yields of variable domain of the heavy chain antibodies produced by the endotoxin-free and Escherichia coli systems.

    Article Snippet: The plasmid pET26b(+), a protein expression vector in E. coli , was purchased from Merck Millipore (Burlington, MA, USA).

    Techniques: Produced, Plasmid Preparation